PCR-Based Setup for High-Throughput cDNA Library Sequencing on the ABI 3700™Automated DNA Sequencer
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چکیده
منابع مشابه
PCR-based setup for high-throughput cDNA library sequencing on the ABI 3700 automated DNA sequencer.
Many large-scale sequencing projects are now being conducted or considered that rely on the latest capillary electrophoresis-based automated sequencing system from Applied Biosystems, the ABI 3700 (PE Biosystems, Foster City, CA, USA). Because of the electrokinetic injection protocol and the physical properties of the capillaries and polymer used, reaction setups that have been optimized for t...
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Next-generation sequencing platforms are powerful technologies, providing gigabases of genetic information in a single run. An important prerequisite for high-throughput DNA sequencing is the development of robust and cost-effective preprocessing protocols for DNA sample library construction. Here we report the development of a semi-automated sample preparation protocol to produce adaptor-ligat...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00294bm05